polymerase

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작성자유토피아 조회 112회 작성일 2020-10-11 16:16:53 댓글 0

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How DNA Polymerase Works

This video explains how DNA Polymerase works. It explains the direction of replication of DNA polymerase and it explains what is required for DNA polymerase to work.

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Alhanouf : Why polymerase 3 needs primers to starts replication or adding nucleotides????
Iman Imad : why polymerase 3 need a primer to start replication ?
Lily Heinzel : Very clear, perfect review. Thanks!
Adele : Thankyou so much, you're a god.
Faya Raheeb : thank uuuuu

PCR - Polymerase Chain Reaction (IQOG-CSIC)

PCR technique (Polymerase Chain Reaction), Animation.
It is a technique used to make multiple copies of a DNA segment of interest, generating a large amount of copies from a small initial simple.
Amplification of DNA segments makes possible the detection of pathogenic virus or bacteria, identification of individuals (DNA fingerprinting), and several scientific research involving DNA manipulation.
Spanish version:


This video has been produced in the Institute of General Organic Chemistry of the CSIC (IQOG-CSIC), Spain, by Guillermo Corrales, as part of its task for promoting Science Communication and may be freely used for educational and science popularization purposes.
Canal Divulgación. Divulgación científica.
Instituto de Química Orgánica General (QOG) CSIC
Madrid, Spain
http://www.youtube.com/user/CanalDivulgacion
Ibad's Biology :

Zaish Khazi :
sewar barakat : فيديو رائع جزاك الله الخير
Muhammad Awais : Thanks
fatiha aoumri : thank you for the video .it is amazing

What is Polymerase Chain Reaction? | PCR Explained

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How does PCR work and what is it used for? Learn all about a polymerase chain reaction in this 2 Minute Classroom video.

Thanks for stopping by, this is 2 Minute Classroom and today we are talking about Polymerase Chain Reaction, or PCR as it’s more commonly known. First, let’s talk about what PCR is and how it works, and then I’ll give some examples of what PCR is used for.

PCR is a technique used to amplify or copy small specific segments of DNA.

For PCR to be successful, you need the DNA sample you want copied, DNA primers to identify the specific sequence of DNA you want to amplify, lots of raw nucleotides to form the DNA copies, and a heat resistant DNA polymerase to make the new copies. This all goes into a special stable solution.

Once all the ingredients are in, you run the solution through many temperature cycles until you reach the amount of DNA copies needed.

There are three main steps to this cycle.

Step 1 - Denaturation

The solution is heated allowing complementary DNA strands to separate so that all the DNA in solution is single stranded.

Step 2 - Annealing

The solution is cooled so that the DNA primers can bind to the specific region of DNA you are interested in.

Step 3 - Extension

The temperature is raised so that the DNA polymerase can bind and perform the necessary operation of synthesizing new DNA strands.

This cycle is repeated about 30 times, takes about 3 hours and creates over one billion copies.

So what is PCR actually used for?

PCR has many research based and practical applications.

It’s used for DNA mapping, like the Human Genome Project.

It’s used to check sections of DNA for genetic testing to determine potential disorders.

Companies like 23andMe and Ancestroy.com use PCR for to run personal DNA tests.
And my favorite application for PCR is in forensics. Whenever a crime is committed, the perpetrator almost always leaves their DNA at the crime scene. This could be hair, semen, skin, or blood. Investigators search for this evidence and thanks to PCR, can amplify the DNA and compare it to the DNA of suspects. This genetic information is then put into the FBIs DNA databade, CODIS

But it gets even better. Investigators have been able to take DNA evidence from crimes committed before CODIS was put together and run it through the current system. This has solved many cold cases and also exonerated prisoners who had been wrongfully accused, even some that were on death row.

Needless to say, PCR is one the most important scientific advances ever. And now you know why.

If you have additional questions I’ll do my best to answer those in the comments below.

Don’t forget to check out my other great videos and I’ll catch you next time.
Mehar Taimoor : 2 Minutes !! Wow . Took classes for almost half a semester and never understood the concept. It took you 2 minutes to explain it! Brilliant :)
이승한 : what nucleotides do?
mauricio xavier : That was so good!
Juan Mahaganti : There are times when we think PCR is needed most to catch lethal homo sapiens
cam luong : So in others words the Covid test is used to test for our DNA. Why want our DNA??? To understand it and produce rna vaccines to mutant us to become evil like them now.

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#polymerase

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